Building of Parhyale Hawaiensis Model

Arthropods are the most numerous and diverse animals on earth. Among them, pancrustaceans are an ancient and morphologically diverse group that includes a wide range of aquatic and semiaquatic crustaceans as well as insects that emerged from crustacean ancestors and colonized most terrestrial habitats. Among insects, Drosophila stands out as one of the most powerful animal models, making significant contributions to our understanding of development, physiology, and behavior. Given these properties, crustaceans provide fertile ground for exploring biodiversity through comparative studies. Recent advances in genetic manipulation and genome sequencing are paving the way for a new generation of research organisms. The marine amphipod Parhyale hawaiensis is the most established crustacean system available, offering year-round access to developmental stages, transgenic tools, genomic resources, and established genetics and imaging methods. Furthermore, Parhyale is easy to maintain and provides a large number of embryos suitable for injection, dissection, and in vivo imaging.

Fig 1. Parhyale cell lineage and development. (Sun D A, et al., 2019)

Our Services

The crustacean amphipod Parhyale hawaiensis has emerged as an attractive experimental model for addressing longstanding questions in biology, including cell fate specification, segmentation, neurogenesis, organ morphogenesis, and regeneration.

• Building of regeneration model
• Building of sensory biology model
• Building of chronobiology model
• Building of ecotoxicology model
• Building of animal development model
• Parhyale embryo live imaging
• Genome editing in Parhyale embryos by CRISPR/Cas
• Parhyale blastomere ablation and isolation
• Gene expression studies of Parhyale embryos
• A comparative genomic analysis of the Parhyale genome
• Parhyale embryo microinjection and transgenesis
• Dissection, fixation, antibody staining, and hybridization of Parhyale embryos

Highlights of Our Parhyale Hawaiensis Modeling Platform

• Our scientists have developed a detailed staging system to characterize the entire Parhyale embryogenesis, as well as robust protocols for in situ collection and fixation of all embryo stages for hybridization to study mRNA localization and immunohistochemistry to study protein localization.
• Well-established techniques for the transient and transgenic introduction of recombinant genetic material, and the availability of endogenous promoters allow for a variety of gene misexpression experiments.
• Targeted inhibition of gene function is possible using RNAi or morpholino approaches.
• Important data for comparative studies aimed at understanding evolution of cis-regulation will be generated by directed genome sequencing.

CD BioSciences is a professional service provider for the marine biology industry. Our Parhyale hawaiensis model building services can ensure that the most suitable methods and techniques are selected for your project. We provide our customers with the most precise ingredient data and highly informed process expertise. Our team of biomass experts plays a key role in the formulation, optimization and commercial evaluation of biomass value-added processes in industry and academia. If necessary, please feel free to contact us.

Reference

• Sun D A, Patel N H. The amphipod crustacean Parhyale hawaiensis: An emerging comparative model of arthropod development, evolution, and regeneration[J]. Wiley Interdisciplinary Reviews: Developmental Biology, 2019, 8(5): e355.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).