Fish Acute Toxicity Test

Acute toxicity tests are short-term tests designed to measure the effects of toxic substances over the short life cycle of aquatic species. The acute toxicity test assessed the effect on survival within 24 to 96 hours. Fish are often used as model organisms for these tests because they have sensitive respiratory systems that make them highly susceptible to waterborne toxins. There are a variety of standardized testing methods available for determining the acute toxicity of chemicals to fish, each with its own advantages and limitations. At CD BioSciences we offer comprehensive fish acute toxicity characterization services using a range of different testing methods to ensure the most accurate and reliable results possible. Some of the fish we tested included Pimephales promelas, Oncorhynchus mykiss, Lep-omis macrochirus, Ictalurus punctatus, Cyprinodon var-iegatus.

Our Services

At CD BioSciences, we offer comprehensive characterization services for fish acute toxicity using a range of different testing methods to ensure the most accurate and reliable results possible. we pride ourselves on using cutting-edge technology and advanced scientific techniques to provide the most accurate and reliable results possible. Our testing capabilities include:

Toxicity reduction evaluations
Toxicity identification evaluations
Whole effluent toxicity (wet) tests
Toxicity treatability evaluations
Bioassay testing and chemical analyses

Test Organisms

Fish acute toxicity test with a variety of species:

Danio rerio
Pimephales promalas
Cyprinus arpio
Poecilia reticulata
Lepomis macrochirus
Oncorhynchus mykiss
Salmo gairdneri
Oryzias latipes

Endpoints of Fish Acute Toxicity Test

Dose-response studies allow calculation of EC₅₀, LC₅₀, no observed effect concentration and lowest observed effect concentration for mortality.

Course of Fish Acute Toxicity Test

Acute toxicity tests are typically performed using five concentrations, a control, and a vehicle (i.e., solvent) control (if the vehicle is required), typically using 10 to 20 organisms per concentration.
Fish are exposed to the test substance for 96 hours by serial dilution. At the same time, a control test line is run without the test substance.
After 24, 48, 72, and 96 h, record mortality and test conditions (temperature, pH, and oxygen content).
The estimated LC₅₀ (Lethal Concentration for 50% of the fish tested) is calculated from the test results based on the dose-response relationship. Based on the test data, LC₀ (the highest concentration at which all fish survived) and LC₁₀₀ (the lowest concentration at which all fish died) are also estimated.

CD BioSciences is a professional service provider for the marine biology industry. Our fish acute test services can ensure that the most suitable methods and techniques are selected for your project. We provide our customers with the most precise ingredient data and highly informed process expertise. Our team of biomass experts plays a key role in the formulation, optimization and commercial evaluation of biomass value-added processes in industry and academia. If necessary, please feel free to contact us.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).