Fish Sexual Development Test

The fish sexual development test assesses the early life stage effects and potential adverse consequences of putative endocrine-disrupting chemicals such as estrogens, androgens, and steroidogenesis inhibitors on sexual development in fish. In the test, fish were exposed to at least three concentrations of the test substance dissolved in water from newly fertilized eggs to the completion of sexual differentiation approximately 60 days after hatching. Within each treatment level and control group, at least four replicates are recommended. At the end of the test, two core endpoints will be measured for each fish: vitellogenin concentration in head-tail or blood sampling, and the proportion of males, females, sexes and undifferentiated fish by gonadal histology. In fish with genetic sex markers, genetic sex is identified to determine sex reversal in individual fish. The combination of the two core endocrine endpoints, vitellogenin concentration and phenotypic (and possibly genotypic) sex ratio, allows the test to indicate the mode of action of the test chemical.

Our Services

With proven technology, CD BioSciences manufactures and provides high-quality fish sexual development testing services. Depending on what our customers wish to use the test for, CD BioSciences can provide the service in different solutions. For more information, data sheets, and quotes please contact us by email.

• Oryzias latipes sexual development test
• Zebrafish sexual development test
• Three-spined sticklebacks sexual development test
• Gasterosteus aculeatus sexual development test
• Medaka sexual development test

Endpoints of Fish Sexual Development Test

Drugs with androgenic or estrogenic properties as well as antiandrogenic, antiestrogenic, and steroidostatic inhibitory properties can be screened using this assay. The following parameters will be determined on endpoints:

• Vitellogenin
• Sex ratio
• Survival
• Hatching success
• Length
• Body weight
• Abnormal behavior
• Morphological abnormalities
• Secondary sex characteristics

Methods of the Test

• Our test is a long-term study of fish approximately 60 days after hatching.
• Fertilized eggs will be exposed to five different concentrations of chemicals in a flow-through system.
• Prepare and observe four replicates of 30 eggs until juvenile gonad development.

Our Beliefs

• Fish sexual development test enables detection of candidate compounds with androgenic or estrogenic properties as well as antiandrogenic, antiestrogenic and steroidogenesis inhibitory properties.
• Reduce researcher wasted time and reduce costs with our superior biotechnology. Our assays are applicable to a wide range of compounds regardless of the mode of action, volatility, solubility and hydrophobicity.

CD BioSciences is a professional service provider for the marine biology industry. Our fish sexual development test services can ensure that the most suitable methods and techniques are selected for your project. We provide our customers with the most precise ingredient data and highly informed process expertise. Our team of biomass experts plays a key role in the formulation, optimization and commercial evaluation of biomass value-added processes in industry and academia. If necessary, please feel free to contact us.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).