Isolation and Purification of Microcystins

Preparative reversed-phase liquid chromatography was successfully used to purify two microcystins (microcystin LR and microcystin LA) from cyanobacterial process waste. Isolation protocols included lyophilized cells by methanol extraction, isolation and concentration by solid-phase extraction, and purification by reversed-phase high-performance liquid chromatography¹. Various techniques are currently available for the purification of microcystins. These methods not only enable accurate quantification of microcystins in a sample but also provide detailed information about the toxin variants present. The efficiency of any technique used for microcystin purification will be affected by the method used to extract the toxin from the sample. Another important factor in microcystin purification is the requirement for sample concentration. Most analytical methods are not sensitive enough to detect trace levels of microcystins in water and can be affected by co-eluting compounds, especially in environmental or biological samples. Preconcentration with C18 or immunoaffinity SPE is often necessary for the accurate detection and purification of microcystins in these complex samples.

Our Services

CD BioSciences has a complete purification suite for clarification and ultrafiltration process steps. With innovative products that work seamlessly at all scales and many years of application knowledge and support, our purification services can help you meet the complex challenges when you strive in a microcystins project. Our services are listed as follows, but are not limited to:

• Extraction of microcystins
• Concentration and clean-up of microcystins
• Preconcentration of microcystins
• Purification of microcystins
• Flash chromatography purification
• Gel filtration chromatography purification
• Ion exchange chromatography purification
• Liquid chromatography purification
• Alternative analytical methods for microcystins
• Electrospray mass spectrometry analysis of microcystin fractions
• Whole organism bioassays
• Immunological assays including ELISA, immunoprotein phosphatase inhibition assay, immunoaffinity chromatography, and a lateral flow "strip" assay
• Solid-phase extraction (SPE)

Key Features of Our Purification Services

• Allow high flow rate
• Concentrate samples
• High yield of microcystins purification
• Buffers are nondenaturing

Customer Notice

Due to the different types of microcystins samples, the sample volume and requirements for transportation conditions of each test item are also different. For more information, please view the details page for each test item. The analysis service has no restrictions on microcystins species. For varieties or rare varieties with special requirements, please contact our full-time technical staff to determine the sample information.

CD BioSciences is a professional service provider for the marine biology industry. Our isolation and purification services of microcystins can ensure that the most suitable methods and techniques are selected for your project. We provide our customers with the most precise ingredient data and highly informed process expertise. Our team of biomass experts plays a key role in the formulation, optimization and commercial evaluation of biomass value-added processes in industry and academia. If necessary, please feel free to contact us.

Reference

• Ramanan S, Tang J, Velayudhan A. Isolation and preparative purification of microcystin variants[J]. Journal of Chromatography A, 2000, 883(1-2): 103-112.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).