Isolation of Biosurfactant Producing Marine Bacteria

Marine biosurfactants are extracellular amphiphilic compounds produced by bacteria, yeast and fungi, especially when they are grown on hydrophobic substrates. Biosurfactants are surface-active compounds produced by microorganisms. They reduce surface and interfacial tension by accumulating at the interface of immiscible fluids, thereby increasing the solubility, bioavailability and subsequent biodegradation of hydrophobic or insoluble organic compounds. Biosurfactants have received extensive attention in the field of environmental remediation processes such as biodegradation, soil washing, and soil flushing. Biosurfactants influence these processes because of their efficacy as dispersants and remediation agents and their environmentally friendly properties, such as low toxicity and high biodegradability. Due to their unique properties and wide range of applications, the identification of new biosurfactant-producing microorganisms is in great demand. At CD BioSciences, our experts in marine biology apply nine different screening methods as criteria for screening biosurfactant-producing microorganisms.

Fig. 1 Marine biosurfactants: biosynthesis, structural diversity and biotechnological applications. (Kubicki S, et al., 2019)

Our Services

At CD BioSciences, our experts compare the reliability and ease of use of hemolysis tests, bacterial adhesion to hydrocarbons (BATH) tests, droplet collapse tests, oil diffusion tests, emulsification tests and surface tension measurements, to screen for bacteria that produce marine biosurfactants. Our services are listed as follows, but are not limited to:

• Surfactant production in marine bacteria
• Isolation of marine biosurfactant producing marine strains
• Culture conditions optimization for production of biosurfactant from marine bacteria
• Screening for biosurfactant production
• Culture conditions optimization for production of biosurfactant from marine bacteria
• Hemolytic assay
• Bacterial adhesion to hydrocarbons (BATH) assay
• Drop collapse assay
• Oil spreading assay emulsification assay
• Surface tension measurement
• Titled glass slide test
• Mulsification assay
• Surface tension measurement
• Blue agar plate and hydrocarbon overlay agar assay

Highlights of Our Marine Bacteria Isolation Services

• Scientific team with extensive experience in handling marine microorganisms
• Fully customizable designs to meet each specific need
• Advanced platform to ensure optimal results for every step
• Fast turnaround time and reasonable price
• Reliable and up-to-date analysis reports
• Friendly, expert advice available

Provided by Customer

Please let us know the details of your specific marine microbial screening service, and we can provide customized solutions for different projects.

Results and Reports

• Gene sequencing data
• Custom analysis report
• Experimental procedure
• MS raw data files and MS data quality checks

CD BioSciences is a professional service provider for the marine biology industry. Our marine bacteria isolation services can ensure that the most suitable methods and techniques are selected for your project. We provide our customers with the most precise ingredient data and highly informed process expertise. Our team of biomass experts plays a key role in the formulation, optimization and commercial evaluation of biomass value-added processes in industry and academia. If necessary, please feel free to contact us.

Reference

• Kubicki S, Bollinger A, Katzke N, et al. Marine biosurfactants: biosynthesis, structural diversity and biotechnological applications[J]. Marine drugs, 2019, 17(7): 408.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).