Marine Microbial Bioburden Testing

Over the past 40 years, the abundance of marine microbial populations has been estimated using flow cytometry (FCM). The technique exploits the inherent properties of cells and fluorescent probes to detect particles in fluid streams, which are then classified into microbiomes based on their properties. Phytoplankton and achromatic marine bacteria have been enumerated using FCM in habitats ranging from the open ocean to coastal regions, as well as in laboratory cultures. Numerous studies have pointed to the ecological importance of viruses in marine microbial food webs, and researchers have used a variety of culture and microscopic techniques to estimate virus abundance in natural waters. Algal virus and phage culture techniques include plaque counting and most probable number techniques. The advantage of these tests is that they only detect viruses that are infectious to a specific host.

Our Services

CD BioSciences has established a proven marine microbial platform for a variety of applications. Our team of dedicated experts has developed a proven validation methodology for the testing process. We'll help you design a suitable testing program to identify harmful marine microorganisms in your products.

• Bioburden testing methods developed and validated for each individual marine bioproduct, ensuring optimal accuracy and repeatability of marine microbial load testing
• Controlled test documentation is issued to customers for each marine bioburden method developed, ensuring consistent testing throughout the marine product life cycle
• Bioburden methods designed to meet each client's needs
• Product warning and/or alert limits Included in individual bioburden methods

Our Marine Microbial Bioburden Methods

The methods used to calculate the microbial content of a product include:

• Plate Counting (TPC) Method
  CD BioSciences offers both inverted plate and diffusion plate methods. The two methods are the same in principle, but slightly different in technique.
• Most Probable Number (MPN) Method
  It is the least accurate method for microbial enumeration, but may be the most appropriate method for some products with very low bioburdens.
• Membrane Filtration Method
  In the membrane filtration method, we filter the sample through two membrane filters with a pore size not greater than 0.45 μm.

Bioburden testing is used for:

• Medical equipment
• Pharmaceuticals
• Packaging
• Raw materials
• Human tissue
• Animal tissue
• Cosmetics

The Bioburden testing process is listed as follows:

Highlights of Marine Microbial Bioburden Testing

• Validate and control the sterilization process
• Monitoring the quality of marine raw materials, components and packaging
• Highlight manufacturing process weaknesses
• Evaluate the effectiveness of cleaning measures

CD BioSciences is a professional service provider for the marine biology industry. Our marine microbial bioburden test services can ensure that the most suitable methods and techniques are selected for your project. We provide our customers with the most precise ingredient data and highly informed process expertise. Our team of biomass experts plays a key role in the formulation, optimization and commercial evaluation of biomass value-added processes in industry and academia. If necessary, please feel free to contact us.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).