Marine Polyunsaturated Fatty Acid Biosynthesis from Yarrowia Lipolytica

Marine Polyunsaturated fatty acids (MPUFAs), especially omega-3 PUFAs composed of eicosapentaenoic acid (EPA) and its counterpart docosahexaenoic acid (DHA), are considered the group of essential fatty acids for human health significant contribution. The main sources of polyunsaturated fatty acids are fish and plants. Fish oil is unsustainable, harmful, and contaminated with toxic metals such as cadmium, lead, and mercury. They are also unstable and expensive to purify. At the same time, global fish populations are declining, and environmental pollution of marine habitats is causing global problems. Therefore, there is a need to obtain alternative sources that provide sustainable polyunsaturated fatty acids and their inexpensive purification processes. There has been considerable interest in the production of polyunsaturated fatty acids from more sustainable, safer and more economical alternative sources. Some microorganisms, especially deep-sea bacteria, can produce polyunsaturated fatty acids. Marine bacteria, especially the genera Shewanella, Photobacter, Colwellia, Moritella, Psychromonas, Vibrio and Alteromonas are found to be one of the major microbial producers of polyunsaturated fatty acids.

Fig. 1 Enzymatic Processing of Omega 3 Long Chain Polyunsaturated Fatty Acid Oils. (Kahveci D, et al., 2015)

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Marine bacteria are involved in PUFA production, but yields remain low. Some metabolic engineering work in Escherichia coli, Synechococcus, Lactococcus lactis, and Pseudomonas putida aimed to increase PUFA production, but the rates, titers, and yields of these organisms are still not sufficient for commercial purposes. At CD BioSciences, our scientists use Yarrowia Lipolytica to produce PUFAs. Yarrowia Lipolytica is the only metabolically engineered yeast available today that can produce sufficient quantities of PUFAs as a commercial product.

• Polyunsaturated fatty acids biosynthesis from Yarrowia Lipolytica
• Polyunsaturated fatty acids analysis
• Polyunsaturated fatty acids purification
• Structure identification of polyunsaturated fatty acids

Strategies for Increasing PUFA Production in Marine Bacteria

• Cerulenin treatment
  Cerulenin is an antifungal compound and a specific condensase inhibitor. Cerulenin was found to inhibit MUFA synthesis, but not PUFA synthesis in M. marina.
• Nitrogen supplementation and limiting the carbon source in growth media
  Nitrogen supplementation, such as tryptone and yeast extract, increases EPA and DHA production, respectively.
• Decreasing the cultivation temperature
  Growth temperature is an important factor affecting the yield of polyunsaturated fatty acids. P. profundum SS9 produces large amounts of polyunsaturated fatty acids when cultured at low temperature.

Polyunsaturated Fatty Acids Analysis

• Absolute quantification: using external standard method for quantification, internal standard correction, qualitative and quantitative more accurate.
• High sensitivity: We use liquid chromatography combined with triple quadrupole mass spectrometer for unsaturated fatty acid analysis services.

CD BioSciences is a professional service provider for the marine biology industry. Our polyunsaturated fatty acids synthesis services can ensure that the most suitable methods and techniques are selected for your project. We provide our customers with the most precise ingredient data and highly informed process expertise. Our team of biomass experts plays a key role in the formulation, optimization and commercial evaluation of biomass value-added processes in industry and academia. If necessary, please feel free to contact us.

Reference

• Kahveci D, Wei W, Xu X. Enzymatic Processing of Omega 3 Long Chain Polyunsaturated Fatty Acid Oils[J]. Current Nutrition & Food Science, 2015, 11(3): 167-176.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).