Purification of Algal Extracts

Algae are lower organisms that grow in the ocean and contain other auxiliary pigments such as chlorophyll. Currently, about 70 kinds of algae in the sea can be food by humans, such as seaweed, stone lily, kelp, and so on. Generally, the protein content of green algae and red algae is higher than that of brown algae. Most brown algae used for industrial development have a protein content of less than 15% (dry weight), and some green algae have a protein content of between 10% and 26%. The algae with higher protein content are red algae, and some species of red algae have a protein content of 47%, which is higher than the protein content of soybeans. Algae are rich in proteins such as phycocyanin, allophycocyanin, and phycoerythrin. Algal proteins can be used as natural pigments in industries such as food, cosmetics, dyes, etc., and can also be made into fluorescent reagents for clinical medical diagnosis and research fields such as immunochemistry and bioengineering. In addition, it can also be made into food and medicine for medical care and has a wide range of applications and high development and utilization value.

Fig. 1 Mechanism of disease resistance in plants, ABA: Abscisic acid, AOS: alleneoxide synthase, APOX: ascorbate peroxidase, CHIT: chitinases, CYP: cytochrome P450, ET: ethylene, ETR: ethylene receptor, GPOX: guaiacol peroxidase, IAA: indole-3-acetic acid, JA: Jasmonic acid, LOX: Lipoxygenase, PAL: Phenylalanine ammonia lyase, POD: peroxidase, PPO: Polyphenoloxydase, PR: Pathogenesis related, SA: Salicylic acid, TF: Transcription factors. (Agarwal P K, et al., 2021)

Our Services

CD BioSciences is a global leader in the supply of natural seaweed extracts to the food ingredients, pharmaceutical, nutraceutical, and cosmetic industries. All of our algal purification services are performed to the highest standards. We test the water quality in all harvest areas and dry the seaweed using our state-of-the-art drying system to ensure the algae's nutrients remain intact during the drying process.

• Characterization of algal proteins by electrophoresis
• Identification of bioactive protein from algae
• Validation of bioactive properties
• Purification of bioactive protein from algae

Our Extraction and Purification Methods of Algal Protein

• Aqueous solution, enzymatic extraction
  The aqueous solution of dilute salt and buffer system has good protein stability and a high solubility and is the most commonly used protein extraction solvent.
• Repeated freeze-thaw method
  The freeze-thaw method means that cells can rupture due to the formation of ice crystals in the cells and the increase in the salt concentration in the remaining liquid during repeated freezing and thawing.
• Ultrasonic fragmentation extraction method
  Our swelling method extracts phycoerythrin with high yield and purity.
• High-pressure homogenization
  We provide a high-pressure homogenization method suitable for large-scale separation and purification of algal proteins in industry.
• Salting out
  Our scientists initially purify the crude extract by a two-step salting-out procedure to obtain the purified protein from the algae.

CD BioSciences is a professional service provider for the marine biology industry. Our algal purification services can help you determine the value of your sample and its best use. We provide our customers with the most precise ingredient data and highly informed process expertise. Our team of biomass experts plays a key role in the formulation, optimization and commercial evaluation of biomass value-added processes in industry and academia. If necessary, please feel free to contact us.

Reference

• Agarwal P K, Dangariya M, Agarwal P. Seaweed extracts: Potential biodegradable, environmentally friendly resources for regulating plant defence[J]. Algal Research, 2021, 58: 102363.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).