Qualitative Analysis of Marine Biosurfactant

Marine biosurfactants are widely used in consumer, industrial, agricultural and pharmaceutical markets in a wide variety of products such as pesticides, detergents, petroleum and cosmetics. Qualitative analysis of surfactants can be difficult due to the diversity of surfactants and the complexity of the sample matrix. Although high performance liquid chromatography (HPLC) is the method of choice and most commonly used for surfactant analysis, choosing the right method for a specific application is always challenging. For example, existing HPLC methods do not provide optimal separation of anionic, nonionic, cationic, and amphoteric surfactants in a single analysis. CD BioSciences' surfactant analysis capabilities range from simple tests involving one technique to protocols involving multiple analytical techniques. Surfactant testing expertise covers a range of capabilities including NMR, mass spectrometry, LC-MS, HPLC and GC-MS. Depending on the nature or concentration of the surfactant, one or several methods may be employed. CD BioSciences provides qualitative analysis of marine biosurfactant for customers.

Fig. 1 A summary of potential approaches for applications of biosurfactants in future outbreak prevention and control. (Çelik P A, et al., 2020)

Our Services

Complex surfactant formulations can contain dozens of ingredients with widely varying concentration levels, requiring a multidisciplinary approach. A coordinated approach that brings together skills in extraction, separation science and spectroscopy may be required. CD BioSciences has the expertise needed in the field of surfactants.

• Qualitative analysis of marine biosurfactant
• Qualitative screening of cationic surfactants
• Qualitative screening of anionic surfactants
• Qualitative screening of nonionic surfactants
• Elements qualitative analysis of marine biosurfactant
• Drop-collapse assay
• Oil spreading assay
• Microplate assay
• Emulsion index (E₂₄)
• Surface tension measurements
• Confocal microscopy of the emulsion
• Emulsion stability studies
• BATH assay
• Determination of critical micelle concentration (CMC)
• Kinetics of biosurfactant production
• Characterization of biosurfactant by thin layer chromatography
• Chemical identification of biosurfactant
• Bioinformatic analysis of the genome

Categories of Marine Biosurfactant Qualitative Analysis

• Non-Ionic Surfactant Analysis
• Alcohol or Phenolic initiated alkoxylates (Butanol ethoxylates, Nonyl phenol ethoxylates)
• PEG esters (Adipic ethoxylate esters)
• Anionic Surfactant Analysis
• Aliphatic and aromatic Sulphonates and Pulphates
• Phosphate Esters
• Cationic Surfactant Analysis
• Ester quats
• Alkyl amine quaternaries
• Zwitterionic Surfactant Analysis
• Betaine Cocamidopropyl betains

Marine Biosurfactant Analysis Capabilities

• Comprehensive portfolio of classical chemical methods for analysis
• MALDI-TOF MS, matrix-assisted laser desorption ionization time-of-flight mass spectrometry
• Other mass spectrometry instruments
• Chromatography, including gel permeation chromatography (GPC)
• Surface tension characterization
• Product identification

CD BioSciences is a professional service provider for the marine biology industry. Our qualitative analysis services of marine biosurfactant can ensure that the most suitable methods and techniques are selected for your project. We provide our customers with the most precise ingredient data and highly informed process expertise. Our team of biomass experts plays a key role in the formulation, optimization and commercial evaluation of biomass value-added processes in industry and academia. If necessary, please feel free to contact us.

Reference

• Çelik P A, Manga E B, Çabuk A, et al. Biosurfactants' potential role in combating COVID-19 and similar future microbial threats[J]. Applied Sciences, 2020, 11(1): 334.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).