Quantitative Analysis of Marine Microbiome Community

Microbiome analysis, as a core technical means to obtain information on all microbial species and abundances in the marine environment, has been widely used in medicine, environment, agriculture, aquaculture, and other fields and has shown important application potential. The acridine orange direct count (AODC) method is one of the most commonly used methods to determine the total number of bacteria in water samples. In the AODC method, the fluorescence emitted by the cells can be observed using an epi-fluorescence microscope. At present, the process of measuring the number, volume, and biomass of bacteria in the water is automated by connecting an epi-fluorescence microscope with an image analyzer and a computer. Since no culture method is suitable for all marine bacteria, and the actual total viable bacterial count in the sample is not grown on the plate, this method can only count the number of marine bacteria. Quantitative microbiome analysis can also overcome the shortcomings of the AODC method by integrating absolute quantification of microbial abundance into next-generation sequencing (NGS) data.

Our Services

CD BioSciences provides comprehensive quality assurance expertise to assist you in culturing, isolating, purifying, and quantifying microbial communities. We have more experienced scientists working on developing new analytical platforms and methods.

• Quantitative microbiome profiling
• Propidium monoazide-based quantitative microbiome profiling
• qPCR-based quantitative microbiome profiling

Our Quantitative Analysis Methods of Microbiome Community

• Direct microscopy counting
  • AODC method
  • Direct microscopic counting of viable bacteria
  • Tetrazolium reduction method
• Culture counting method
  • Coated plate counting
  • Most probable number (MPN) counting method
• Microporous membrane counting method
• Flow cytometry counting method
• High-throughput rRNA gene tag sequencing

Project Design/Advice Assistance

All of CD BioSciences' sequencing services involve a streamlined workflow by a team of specially trained and experienced scientists. Our technical support team will be happy to work with you to confirm your order details.

Why Use Our Microbiome Quantification Service?

• Scientific team with extensive experience in handling marine microorganisms
• Fully customizable designs to meet each specific need
• Advanced platform to ensure optimal results for every step
• Fast turnaround time and reasonable price
• Reliable and up-to-date analysis reports
• Friendly, expert advice available

Customer Notice

• You can provide us with specific information about the microorganisms that need to be produced, and we provide integrated services from cultivation to quantitative analysis.
• You can ship your samples to CD BioSciences' microbiology laboratory, please contact our dedicated staff before sending your samples and make a backup.
• We provide marine microbial quantitative analysis packages, including culture, isolation, and purification of marine microorganisms, biochemical testing, quantification of water quality parameters, fluorescence counting, and high-throughput sequencing.

CD BioSciences is a professional service provider for the marine biology industry. Our quantifying microbial community services can ensure that the most suitable methods and techniques are selected for your project. We provide our customers with the most precise ingredient data and highly informed process expertise. Our team of biomass experts plays a key role in the formulation, optimization and commercial evaluation of biomass value-added processes in industry and academia. If necessary, please feel free to contact us.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).