Unsaturated Fatty Acids Biosynthesis from Thraustochytrium

Thraustochytrium are unicellular fungus-like marine protists that are ubiquitous in marine environments. They significantly contribute to the synthesis of omega-3 polyunsaturated fatty acids (PUFAs), docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), which are essential for growth and reproduction in crustaceans' fatty acid. Thraustochytrium is a unicellular protist belonging to the Labyrinth fungus genus, characterized by the presence of a high lipid content that can replace conventional fatty acids. Thraustochytrium biomass is estimated to exceed planktonic bacteria in both coastal and marine waters. Also, they need sodium to survive, which cannot be replaced by potassium. When Thraustochytrium is cultivated under certain conditions, some Thraustochytrium can have their total weight composed of 15-25% DHA. Scientists have genetically engineered several pathways to increase the production of unsaturated fatty acids in Thraustochytrium by creating mutants, overexpressing genes, and/or introducing knocking effects.

Our Services

CD BioSciences has been providing high-quality services for many years. Customer service, continuous monitoring of market demand, and research and development activities are all key considerations for CD BioSciences in the design and manufacture of unsaturated fatty acids. CD BioSciences provides one-stop service for producing unsaturated fatty acidsfrom Thraustochytrium.

• Thraustochytrium Strain Isolated from Marine
  CD BioSciences collects samples of bacterial beads in medium and observes them using light microscopy.
• Fermentation of Thraustochytrium to Produce Unsaturated Fatty Acids
  CD BioSciences fluorescent staining technique to estimate the cell number of Thraustochytrium. The technique involves a reaction between a fluorescent dye (acriflavine), a sulfated cell wall, and its nucleus.
• Thraustochytrium Strain Analysis Services
• Morphologic and genetic identification
• DNA extraction and amplification of the 18S rRNA gene
• Sequencing and molecular phylogenetic analysis
• Genome sequence annotation
• Gene ontology analysis
• Phylogenic analysis
• Gene family analysis
• Extraction of Unsaturated Fatty Acids
  CD BioSciences completed fatty acid profiles with 50 mg of frozen biomass using a direct transesterification reaction. CD BioSciences measures unsaturated fatty acid content gravimetrically.
• Determination and Analysis of Unsaturated Fatty Acids
  Unsaturated fatty acid qualitative studies are performed using Oil Red O staining, a fat-soluble diazo dye.

Why use our unsaturated fatty acids biosynthesis service?

• A scientific team with extensive experience in handling marine microorganisms, we use gas chromatography (GC) for the determination of unsaturated fatty acids.
• Fully customizable biosynthesis process designs to meet each specific need.
• Advanced platform, we use gene ontology analysis, phylogenic analysis and gene family analysis to ensure the accuracy of Thraustochytrium analysis.
• Fast turnaround time and reasonable price.
• Reliable and up-to-date analytical reports.
• Provide friendly expert advice.

CD BioSciences is a professional service provider for the marine biology industry. Our unsaturated fatty acids biosynthesis services can ensure that the most suitable methods and techniques are selected for your project. We provide our customers with the most precise ingredient data and highly informed process expertise. Our team of biomass experts plays a key role in the formulation, optimization and commercial evaluation of biomass value-added processes in industry and academia. If necessary, please feel free to contact us.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).